B-NDG Kit W41 mice
| Strain Name |
NOD.CB17-Prkdcscid Il2rgtm1Bcgen Kittm1(V831M)Bcgen/Bcgen
|
Common Name |
B-NDG Kit W41 mice |
| Background |
B-NDG |
Catalog number | 112914 |
| Aliases | Human: PBT, SCFR, C-Kit, CD117, MASTC | ||
|
NCBI Gene ID |
3815 | ||
Description
- C-Kit is expressed in various cells within the body, and it is significantly expressed in stem cells, progenitor cells, and other cells with self-renewal capabilities. In normal bone marrow, c-Kit is expressed by hematopoietic stem cells and plays a crucial role in self-renewal and differentiation into various blood cells. After binding with its ligand SCF, c-Kit undergoes autophosphorylation, thereby activating downstream signaling pathways and participating in cellular processes such as cell proliferation, differentiation, survival, adhesion, motility, and angiogenesis.
- Gene editing strategy: The 831 acid amino Val of Kit gene was mutated as Met in B-NDG Kit W41 mice.
- mRNA expression analysis: Mouse Kit mRNA were detectable in B-NDG mice (+/+) and B-NDG Kit W41 mice (Mut/+). Sequence assays showed the B-NDG Kit W41 mice (Mut/+) consists of a G to A point mutation at amino acid 831.
- Application: This product can accept human hematopoietic stem cell transplantation without irradiation, making it suitable for research in the fields of blood and immunity.
mRNA expression analysis
Strain specific analysis of Kit mRNA expression in B-NDG mice and B-NDG Kit W41 mice by RT-PCR. Spleen and lung RNA were isolated from B-NDG mice (+/+) and heterozygous B-NDG Kit W41 mice (Mut/+), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Kit primers. Mouse Kit mRNA were detectable in B-NDG mice (+/+) and B-NDG Kit W41 mice (Mut/+). Sequence assays showed the B-NDG Kit W41 mice (Mut/+) consists of a G to A point mutation at amino acid 831.
Protein expression analysis-Neutrophils
Strain specific KIT expression analysis in B-NDG mice and B-NDG Kit W41 mice by flow cytometry. Bone marrow was collected from B-NDG mice and homozygous B-NDG Kit W41 mice (Mut/Mut). Protein expression was analyzed with species-specific anti-KIT antibody (Biolegend, 105811). mKIT was detectable in neutrophils of B-NDG mice and homozygous B-NDG Kit W41 mice.
Strain specific KIT expression analysis in B-NDG mice and B-NDG Kit W41 mice by flow cytometry. Bone marrow was collected from B-NDG mice and homozygous B-NDG Kit W41 mice (Mut/Mut). Protein expression was analyzed with species-specific anti-KIT antibody (Biolegend, 105811). mKIT was detectable in DCs of B-NDG mice and homozygous B-NDG Kit W41 mice.
Strain specific KIT expression analysis in B-NDG mice and B-NDG Kit W41 mice by flow cytometry. Bone marrow was collected from B-NDG mice and homozygous B-NDG Kit W41 mice (Mut/Mut). Protein expression was analyzed with species-specific anti-KIT antibody (Biolegend, 105811). mKIT was detectable in CD11b+ cells of B-NDG mice and homozygous B-NDG Kit W41 mice.
Frequency of leukocyte subpopulations by flow cytometry. Splenocytes, blood cells and bone marrow cells were isolated from B-NDG mice and homozygous B-NDG Kit W41 mice (n=3, 8-week-old). Flow cytometry analysis of the Splenocytes, blood cells and Bone marrow cells were performed to assess the frequency of leukocyte subpopulations. Percentages of T cells, B cells, NK cells, DCs, granulocytes, monocytes, macrophages in B-NDG Kit W41 mice were similar to those in B-NDG mice, demonstrating that mutation of Kit does not change the frequency or distribution of these cell types in spleen(A), blood(B) and bone marrow(C). Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001.
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